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Supplementary material (ESI) for Chemical

supplementary material (esi) for chemical communications this journal is © the royal society of chemistry 2003 supplementary inf
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Supplementary material (ESI) for Chemical Communications
This journal is © The Royal Society of Chemistry 2003
Supplementary Information
Cell uptake assays with peptoids 3ac
Cells were grown in DMEM medium supplemented with 4 mM glutamine, 10%
FCS and 100units/ml penicillin/streptomycin until 80% confluency.
Cells were suspended using trypsin/EDTA and counted. Cells were then
seeded in 96 well plates at 2x104 cells per well and incubated
overnight (or required time period).
Cells were washed with warm PBS buffer. Compounds 3a3c were mixed
with serum free medium (SFM) to final concentrations of 0.1 M, 1 M,
10 M, and 20 M. To each well 200 l of the various concentrations of
3a3c were added and incubated at the required temperature (4C, 20C,
37C) and for the required time period. Each concentration was
performed in triplicate. The internalisation of free fluorescein,
under the same conditions, was tested simultaneously. After
incubation, cells were washed twice with PBS and the cells analysed
using fluorescence microscopy and FACS analysis.
(a) (b)

Figure

Analysis of cells by fluorescence microscopy

(a) HEK293T (human embryonic kidney) cells, general transmission image
at low magnification
(b) 3b (n 5) 10M, 37ºC for 6h, fluorescent image at low
magnification
The effect of NaN3 on cellular uptake with peptoids 3ac

Cells were preincubated with 0.5% sodium azide in SFM for 30min. The
medium was then removed and 200l of compound 3 (10 m in SFM) in the
presence and absence of 0.5% sodium azide was added. The cells were
incubated for 4 hours. The cells were fixed and analysed by
fluorescence microscopy.

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